In the absence of effective real lane change accident data, the potential conflict forms of vehicles with surrounding vehicles at different stages of complete lane change process are analyzed based on traffic conflict theory. Through the derivation of microscopic conflict risk indicators, the extraction of macroscopic lane change risk features and the analysis on systematic lane change risk, a comprehensive quantification method of lane change risk is established with concurrent considerations on the possibility and severity of potential collisions. An application test is conducted to analyze the comprehensive risk quantification method using the natural driving trajectory data set. The results show that the quantification method proposed can objectively and reasonably reflect the risk features of lane change of different types and locations, providing a new idea for the decision-making and planning of safe lane change of intelligent vehicles.